NpgRJ_nmeth_793 837..843
نویسندگان
چکیده
Light-sensitive ‘caged’ molecules provide a means of rapidly and noninvasively manipulating biochemical signals with submicron spatial resolution. Here we describe a new optical system for rapid uncaging in arbitrary patterns to emulate complex neural activity. This system uses TeO2 acousto-optical deflectors to steer an ultraviolet beam rapidly and can uncage at over 20,000 locations per second. The uncaging beam is projected into the focal plane of a two-photon microscope, allowing us to combine patterned uncaging with imaging and electrophysiology. By photolyzing caged neurotransmitter in brain slices we can generate precise, complex activity patterns for dendritic integration. The method can also be used to activate many presynaptic neurons at once. Patterned uncaging opens new vistas in the study of signal integration and plasticity in neuronal circuits and other biological systems.
منابع مشابه
ScienceDirect - Journal of Chromatography B, Volume 850, Issues 1-2, Pages 1-588 (1 May 2007)
= Full-text available = Non-subscribed What does this mean? Articles in Press Volume 857 (2007) Volume 856 (2007) Volume 855 (2007) Volume 854 (2007) Volume 853 (2007) Volume 852 (2007) Volume 851 (2007) Volume 850 (2007) Volume 850, Issues 1-2 pp. 1-588 (1 May 2007) Volume 849 (2007) Volume 848 (2007) Volume 847 (2007) Volume 846 (2007) Volume 845 (2007) Volume 844 (2006) Volume 843 (2006) Vol...
متن کاملScienceDirect - Journal of Chromatography B, Volume 846, Issues 1-2, Pages 1-390 (1 February 2007)
Articles in Press Volume 849 (2007) Volume 848 (2007) Volume 847 (2007) Volume 846 (2007) Volume 846, Issues 1-2 pp. 1-390 (1 February 2007) Volume 845 (2007) Volume 844 (2006) Volume 843 (2006) Volume 842 (2006) Volume 841 (2006) Volume 840 (2006) Volume 839 (2006) Volume 838 (2006) Volume 837 (2006) Volume 836 (2006) Volume 835 (2006) Volume 834 (2006) Volume 833 (2006) Volume 832 (2006) Volu...
متن کاملScienceDirect - Journal of Chromatography B, Volume 858, Issues 1-2, Pages 1-312 (15 October 2007)
= Full-text available = Non-subscribed What does this mean? Articles in Press Volume 858 (2007) Volume 858, Issues 1-2 pp. 1-312 (15 October 2007) Volume 857 (2007) Volume 856 (2007) Volume 855 (2007) Volume 854 (2007) Volume 853 (2007) Volume 852 (2007) Volume 851 (2007) Volume 850 (2007) Volume 849 (2007) Volume 848 (2007) Volume 847 (2007) Volume 846 (2007) Volume 845 (2007) Volume 844 (2006...
متن کاملCNS Drugs 2009;
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